To respond to widespread violence against women and girls by providing psychosocial support and shelter for victims, strengthening youth leadership to raise local awareness of sexual and gender-based violence, and conducting human rights advocacy with local institutions.

To defend and promote the sexual health and rights of transgender people in Nicaragua by conducting human rights education and leadership building initiatives with the transgender community, holding video-forums about gender identity, denouncing human rights violations, and conducting workshops with transgender people on gender identity, sexual rights, stigma and discrimination, and HIV prevention. ANIT also conducts awareness-raising workshops with the staff of public institutions and with government and social leaders.

To reduce sexual and gender-based violence and promote gender equity within indigenous communities by conducting trainings on women's rights and access to justice for victims of gender-based violence and by raising awareness among traditional justice leaders about gender equity.

To advance indigenous youth's rights and leadership by conducting leadership development workshops, establishing a youth council, and developing youth-led advocacy initiatives.

To address poverty and food insecurity by providing low-interest loans and technical assistance to rural farming families, supporting them to run small businesses and diversify their agricultural production.

To work with the indigenous community of Totogalpa to protect indigenous people's collective rights by strengthening traditional administrative structures, increasing communities' capacity to manage their territory, strengthening participation of youth and women in natural resource rights agendas, and conducting trainings with communities on indigenous people's rights.

To protect and respect sex workers' rights through human rights education, sexual and reproductive health trainings, and advocacy with local authorities.

To run educational sessions for LBT women on health and human rights based on the Yogyakarta Principles and to conduct awareness-raising campaigns, hold meetings with municipal authorities to discuss discrimination against LBT women, monitor human rights violations and provide psychosocial support for LBT mothers.